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Combining 3D graphene-like screen-printed carbon electrode with methylene blue-loaded liposomal nanoprobes for phospholipase A2 detection.

ABSTRACT: Phospholipase A2 (PLA2) enzyme could be acted as a unique biomarker for forecasting and diagnosing certain diseases. Therefore, it is important to monitor PLA2 activity in biological and clinical samples. In this work, a simple electrochemical assay for PLA2 activity was developed based on a screen-printed carbon electrode (SPCE) with 3D graphene-like surface. When the PLA2-containing sample was mixed with the nanoprobes, i.e. the electroactive marker methylene blue (MB) encapsulated within nanometer-sized phospholipid liposomes, MB was released and adsorbed/enriched in site onto the surface of SPCE in a micro-cell. The encapsulation and enzymatic release of MB were evaluated using UV-Vis and fluorescence. The peak current due to oxidation of the adsorbed MB on the SPCE was measured by square-wave voltammetry (SWV). The current was directly linear to the PLA2 activity from 5?U/L to 200?U/L with a detection limit of 3?U/L. The same method can also be used for screening PLA2 inhibitors. Thus, the enrichment strategy developed in this work could be a promising signal amplification method for the sensitive and selective detection of PLA2 in biological or clinical samples.

SUBMITTER: Zhang Y

PROVIDER: S-EPMC6413735 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

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Combining 3D graphene-like screen-printed carbon electrode with methylene blue-loaded liposomal nanoprobes for phospholipase A2 detection.

Zhang Yonghua Y Ai Junjie J Dong Yanan Y Zhang Shiyu S Gao Qiang Q Qi Honglan H Zhang Chengxiao C Cheng Zhiliang Z

Biosensors & bioelectronics 20181103

Phospholipase A2 (PLA2) enzyme could be acted as a unique biomarker for forecasting and diagnosing certain diseases. Therefore, it is important to monitor PLA2 activity in biological and clinical samples. In this work, a simple electrochemical assay for PLA2 activity was developed based on a screen-printed carbon electrode (SPCE) with 3D graphene-like surface. When the PLA2-containing sample was mixed with the nanoprobes, i.e. the electroact ...[more]

PMID: 30445300

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Project description:SiO2@TiO2 core-shell nanoparticles were successfully synthesized via a simple, reproducible, and low-cost method and tested for methylene blue adsorption and UV photodegradation, with a view to their application in wastewater treatment. The monodisperse SiO2 core was obtained by the classical Stöber method and then coated with a thin layer of TiO2, followed by calcination or hydrothermal treatments. The properties of SiO2@TiO2 core-shell NPs resulted from the synergy between the photocatalytic properties of TiO2 and the adsorptive properties of SiO2. The synthesized NPs were characterized using FT-IR spectroscopy, HR-TEM, FE-SEM, and EDS. Zeta potential, specific surface area, and porosity were also determined. The results show that the synthesized SiO2@TiO2 NPs that are hydrothermally treated have similar behaviors and properties regardless of the hydrothermal treatment type and synthesis scale and better performance compared to the SiO2@TiO2 calcined and TiO2 reference samples. The generation of reactive species was determined by EPR, and the photocatalytic activity was evaluated by the methylene blue (MB) removal in aqueous solution under UV light. Hydrothermally treated SiO2@TiO2 showed the highest adsorption capacity and photocatalytic removal of almost 100% of MB after 15 min in UV light, 55 and 89% higher compared to SiO2 and TiO2 reference samples, respectively, while the SiO2@TiO2 calcined sample showed 80%. It was also observed that the SiO2-containing samples showed a considerable adsorption capacity compared to the TiO2 reference sample, which improved the MB removal. These results demonstrate the efficient synergy effect between SiO2 and TiO2, which enhances both the adsorption and photocatalytic properties of the nanomaterial. A possible photocatalytic mechanism was also proposed. Also noteworthy is that the performance of the upscaled HT1 sample was similar to one of the lab-scale synthesized samples, demonstrating the potentiality of this synthesis methodology in producing candidate nanomaterials for the removal of contaminants from wastewater.

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Combining 3D graphene-like screen-printed carbon electrode with methylene blue-loaded liposomal nanoprobes for phospholipase A2 detection.

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Combining 3D graphene-like screen-printed carbon electrode with methylene blue-loaded liposomal nanoprobes for phospholipase A<sub>2</sub> detection.

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