Dataset Information

Synthesis and Morphological Control of Biocompatible Fluorescent/Magnetic Janus Nanoparticles Based on the Self-Assembly of Fluorescent Polyurethane and Fe?O? Nanoparticles.

ABSTRACT: Functionalized Janus nanoparticles have received increasing interest due to their anisotropic shape and the particular utility in biomedicine areas. In this work, a simple and efficient method was developed to prepare fluorescent/magnetic composite Janus nanoparticles constituted of fluorescent polyurethane and hydrophobic nano Fe?O?. Two kinds of fluorescent polyurethane prepolymers were synthesized by the copolymerization of fluorescent dye monomers, and the fluorescent/magnetic nanoparticles were fabricated in one-pot via the process of mini-emulsification and self-assembly. The nanostructures of the resulting composite nanoparticles, including core/shell and Janus structure, could be controlled by the phase separation in assembly process according to the result of transmission electron microscopy, whereas the amount of the nonpolar segments of polyurethane played an important role in the particle morphology. The prominent magnetic and fluorescent properties of the Janus nanoparticles were also confirmed by vibrating magnetometer and confocal laser scanning microscope. Furthermore, the Janus nanoparticles featured excellent dispersity, storage stability, and cytocompatibility, which might benefit their potential application in biomedical areas.

SUBMITTER: Li B

PROVIDER: S-EPMC6419061 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

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Synthesis and Morphological Control of Biocompatible Fluorescent/Magnetic Janus Nanoparticles Based on the Self-Assembly of Fluorescent Polyurethane and Fe₃O₄ Nanoparticles.

Li Botian B Shao Wei W Wang Yanzan Y Xiao Da D Xiong Yi Y Ye Haimu H Zhou Qiong Q Jin Qingjun Q

Polymers 20190205 2

Functionalized Janus nanoparticles have received increasing interest due to their anisotropic shape and the particular utility in biomedicine areas. In this work, a simple and efficient method was developed to prepare fluorescent/magnetic composite Janus nanoparticles constituted of fluorescent polyurethane and hydrophobic nano Fe₃O₄. Two kinds of fluorescent polyurethane prepolymers were synthesized by the copolymerization of fluorescent dye monomers, and the fluorescent/magnetic nanoparticles ...[more]

PMID: 30960256

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Project description:Fluorescent imaging agents that can specifically highlight tumor cells could have a significant impact on image-guided tumor removal. Here, fluorescent nanoparticles (NPs) derived from hyaluronic acid (HA) are investigated. HA is a ligand for the receptor CD44, which is a common biomarker present on many primary tumor cells, cancer-initiating cells, and tumor-associated fibroblasts. In addition, a family of enzymes that degrade HA, called hyaluronidases (HYALs), are also overexpressed with increased activity in many tumors. We report the design and development of a panel of targeted imaging agents using the near-infrared (NIR) dye, Cy7.5, that was directly conjugated to hydrophobically-modified HA. Two different molecular weights of HA, 10kDa and 100kDa, and three different degrees of hydrophobic moiety conjugation (0, 10, and 30mol%) were utilized to develop a panel of NPs with variable size that ranged from 50 to 400nm hydrodynamic diameter (HD) depending HA molecular weight, extent of fluorescence quenching (25-50%), kinetics of cellular uptake, and targeting to CD44+ cells. The kinetics and energy-dependence of cellular uptake in breast and prostate cancer cell lines, MDA-MB 231 and PC-3 cells, respectively, showed increased uptake with longer incubation times (at 4 and 8h compared to 1h), as well as uptake at 37°C but not 4°C, which indicated energy-dependent endocytosis. NP uptake studies in the presence of excess free HA showed that pre-treatment of cells with excess high molecular weight (MW) free HA decreased NP uptake by up to 50%, while no such trend was observed with low MW HA. These data lay the foundation for selection of optimized HA-derived NPs for image-guided surgery.Here, hyaluronic acid (HA), a well-studied biomacromolecule, is modified with a near infrared fluorophore and a hydrophobic moiety. The significance of this work, especially for imaging applications, is that the impact of HA molecular weight and the hydrophobic moiety conjugation degree on fluorescence and cell interaction can be predicted. With respect to existing literature, the eventual use of these HA-based NPs is image-guided surgery; thus, we focus on the dye, Cy7.5, for conjugation, which is more NIR than most existing HA literature. Furthermore, HA is a ligand for CD44, which is associated with cancer and tumor microenvironment cells. Systematic studies in this work highlight that HA can be tuned to maximize or minimize CD44 binding.

Dataset Information

Synthesis and Morphological Control of Biocompatible Fluorescent/Magnetic Janus Nanoparticles Based on the Self-Assembly of Fluorescent Polyurethane and Fe?O? Nanoparticles.

Publications

Synthesis and Morphological Control of Biocompatible Fluorescent/Magnetic Janus Nanoparticles Based on the Self-Assembly of Fluorescent Polyurethane and Fe₃O₄ Nanoparticles.

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