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Plasma cell-free DNA: a potential biomarker for early prediction of severe dengue.

ABSTRACT: BACKGROUND:Considerable progress has been made in dengue management, however the lack of appropriate predictors of severity has led to huge number of unwanted admissions mostly decided on the grounds of warning signs. Apoptosis related mediators, among others, are known to correlate with severe dengue (SD) although no predictive validity is established. The objective of this study was to investigate the association of plasma cell-free DNA (cfDNA) with SD, and evaluate its prognostic value in SD prediction at acute phase. METHODS:This was a hospital-based prospective cohort study conducted in Vietnam. All the recruited patients were required to be admitted to the hospital and were strictly monitored for various laboratory and clinical parameters (including progression to SD) until discharged. Plasma samples collected during acute phase (6-48 h before defervescence) were used to estimate the level of cfDNA. RESULTS:Of the 61 dengue patients, SD patients (n?=?8) developed shock syndrome in 4.8 days (95% CI 3.7-5.4) after the fever onset. Plasma cfDNA levels before the defervescence of SD patients were significantly higher than the non-SD group (p?=?0.0493). From the receiver operating characteristic (ROC) curve analysis, a cut-off of?>?36.9 ng/mL was able to predict SD with a good sensitivity (87.5%), specificity (54.7%), and area under the curve (AUC) (0.72, 95% CI 0.55-0.88; p?=?0.0493). CONCLUSIONS:Taken together, these findings suggest that cfDNA could serve as a potential prognostic biomarker of SD. Studies with cfDNA kinetics and its combination with other biomarkers and clinical parameters would further improve the diagnostic ability for SD.

SUBMITTER: Phuong NTN

PROVIDER: S-EPMC6419393 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature

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Plasma cell-free DNA: a potential biomarker for early prediction of severe dengue.

Phuong Nguyen Thi Ngoc NTN Manh Dao Huy DH Dumre Shyam Prakash SP Mizukami Shusaku S Weiss Lan Nguyen LN Van Thuong Nguyen N Ha Tran Thi Ngoc TTN Phuc Le Hong LH Van An Tran T Tieu Thuan Minh TM Kamel Mohamed Gomaa MG Morra Mostafa Ebraheem ME Huong Vu Thi Que VTQ Huy Nguyen Tien NT Hirayama Kenji K

Annals of clinical microbiology and antimicrobials 20190313 1

<h4>Background</h4>Considerable progress has been made in dengue management, however the lack of appropriate predictors of severity has led to huge number of unwanted admissions mostly decided on the grounds of warning signs. Apoptosis related mediators, among others, are known to correlate with severe dengue (SD) although no predictive validity is established. The objective of this study was to investigate the association of plasma cell-free DNA (cfDNA) with SD, and evaluate its prognostic valu ...[more]

PMID: 30871553

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Plasma cell-free DNA: a potential biomarker for early prediction of severe dengue.

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Plasma cell-free DNA: a potential biomarker for early prediction of severe dengue.

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