Reactive oxygen species-induced alterations in H19-Igf2 methylation patterns, seminal plasma metabolites, and semen quality.
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ABSTRACT: Purpose
This study was conducted in order to investigate the effects of reactive oxygen species (ROS) levels on the seminal plasma (SP) metabolite milieu and sperm dysfunction.Methods
Semen specimens of 151 normozoospermic men were analyzed for ROS by chemiluminescence and classified according to seminal ROS levels [in relative light units (RLU)/s/106 sperm]: group 1 (n?=?39): low (ROS?Result(s)The methylation status of H19 and Igf2 was significantly different in specimens with high ROS (P?Conclusion(s)Results showed significant correlation between ROS levels and H19-Igf2 gene methylation as well as semen parameters. These findings are critical to identify idiopathic male infertility and its management through assisted reproduction technology (ART).
SUBMITTER: Darbandi M
PROVIDER: S-EPMC6420547 | biostudies-literature | 2019 Feb
REPOSITORIES: biostudies-literature
Publications
Reactive oxygen species-induced alterations in H19-Igf2 methylation patterns, seminal plasma metabolites, and semen quality.
Darbandi Mahsa M Darbandi Sara S Agarwal Ashok A Baskaran Saradha S Dutta Sulagna S Sengupta Pallav P Khorram Khorshid Hamid Reza HR Esteves Sandro S Gilany Kambiz K Hedayati Mehdi M Nobakht Fatemeh F Akhondi Mohammad Mehdi MM Lakpour Niknam N Sadeghi Mohammad Reza MR
Journal of assisted reproduction and genetics 20181031 2
<h4>Purpose</h4>This study was conducted in order to investigate the effects of reactive oxygen species (ROS) levels on the seminal plasma (SP) metabolite milieu and sperm dysfunction.<h4>Methods</h4>Semen specimens of 151 normozoospermic men were analyzed for ROS by chemiluminescence and classified according to seminal ROS levels [in relative light units (RLU)/s/10<sup>6</sup> sperm]: group 1 (n = 39): low (ROS < 20), group 2 (n = 38): mild (20 ≤ ROS < 40), group 3 (n = 31): moderate (40 ≤ ROS ...[more]
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