Seminal plasma HSPA2 mRNA content is associated with semen quality.
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ABSTRACT: Purpose
Cell-free mRNAs (cfmRNAs) were quantitatively measured in human seminal plasma and its relationship with semen quality was investigated.Methods
Herein, a prospectively, controlled investigation was performed to study seminal plasma HSPA2 and uPA cfmRNA alterations between 21 asthenozoospermic patients and 16 normozoospermic individuals. Standard semen analysis was performed and seminal plasma cfmRNAs content was measured by real-time quantitative PCR. In addition, the regression analysis between seminal plasma cfmRNAs expression and semen parameters was performed.Results
Seminal plasma HSPA2, but not uPA cfmRNA indicated significant difference between normozoospermia and asthenozoospermia men (P?=?0.02444 and 0.07811, respectively). Negative correlation between HSPA2 cfmRNA and sperm motility (R (2)?=?0.213, P?=?0.004) as well as sperm concentration (R (2)?=?0.133, P?=?0.026) were revealed. However, no correlation was found between seminal plasma uPA cfmRNA content and semen parameters.Conclusions
Our data suggest that seminal plasma HSPA2 cfmRNA is different between asthenozoospermic and normozoospermic individuals and it might be an indicator for semen quality.
SUBMITTER: Tian Y
PROVIDER: S-EPMC4974226 | biostudies-literature | 2016 Aug
REPOSITORIES: biostudies-literature
Publications
Seminal plasma HSPA2 mRNA content is associated with semen quality.
Tian Yonghong Y Li Lejun L Zhang Fengbin F Xu Jian J
Journal of assisted reproduction and genetics 20160521 8
<h4>Purpose</h4>Cell-free mRNAs (cfmRNAs) were quantitatively measured in human seminal plasma and its relationship with semen quality was investigated.<h4>Methods</h4>Herein, a prospectively, controlled investigation was performed to study seminal plasma HSPA2 and uPA cfmRNA alterations between 21 asthenozoospermic patients and 16 normozoospermic individuals. Standard semen analysis was performed and seminal plasma cfmRNAs content was measured by real-time quantitative PCR. In addition, the reg ...[more]
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