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Automating multimodal microscopy with NanoJ-Fluidics.

ABSTRACT: Combining and multiplexing microscopy approaches is crucial to understand cellular events, but requires elaborate workflows. Here, we present a robust, open-source approach for treating, labelling and imaging live or fixed cells in automated sequences. NanoJ-Fluidics is based on low-cost Lego hardware controlled by ImageJ-based software, making high-content, multimodal imaging easy to implement on any microscope with high reproducibility. We demonstrate its capacity on event-driven, super-resolved live-to-fixed and multiplexed STORM/DNA-PAINT experiments.

SUBMITTER: Almada P

PROVIDER: S-EPMC6420627 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature

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Automating multimodal microscopy with NanoJ-Fluidics.

Almada Pedro P Pereira Pedro M PM Culley Siân S Caillol Ghislaine G Boroni-Rueda Fanny F Dix Christina L CL Charras Guillaume G Baum Buzz B Laine Romain F RF Leterrier Christophe C Henriques Ricardo R

Nature communications 20190315 1

Combining and multiplexing microscopy approaches is crucial to understand cellular events, but requires elaborate workflows. Here, we present a robust, open-source approach for treating, labelling and imaging live or fixed cells in automated sequences. NanoJ-Fluidics is based on low-cost Lego hardware controlled by ImageJ-based software, making high-content, multimodal imaging easy to implement on any microscope with high reproducibility. We demonstrate its capacity on event-driven, super-resolv ...[more]

PMID: 30874553

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