Ontology highlight
ABSTRACT:
SUBMITTER: Inglis AJ
PROVIDER: S-EPMC6421407 | biostudies-literature | 2019 Mar
REPOSITORIES: biostudies-literature
Inglis Alison J AJ Masson Glenn R GR Shao Sichen S Perisic Olga O McLaughlin Stephen H SH Hegde Ramanujan S RS Williams Roger L RL
Proceedings of the National Academy of Sciences of the United States of America 20190225 11
Cells dynamically adjust their protein translation profile to maintain homeostasis in changing environments. During nutrient stress, the kinase general control nonderepressible 2 (GCN2) phosphorylates translation initiation factor eIF2α, initiating the integrated stress response (ISR). To examine the mechanism of GCN2 activation, we have reconstituted this process in vitro, using purified components. We find that recombinant human GCN2 is potently stimulated by ribosomes and, to a lesser extent, ...[more]