Ontology highlight
ABSTRACT:
SUBMITTER: Harding HP
PROVIDER: S-EPMC6919976 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
Harding Heather P HP Ordonez Adriana A Allen Felicity F Parts Leopold L Inglis Alison J AJ Williams Roger L RL Ron David D
eLife 20191121
The eukaryotic translation initiation factor 2α (eIF2α) kinase GCN2 is activated by amino acid starvation to elicit a rectifying physiological program known as the Integrated Stress Response (ISR). A role for uncharged tRNAs as activating ligands of yeast GCN2 is supported experimentally. However, mouse GCN2 activation has recently been observed in circumstances associated with ribosome stalling with no global increase in uncharged tRNAs. We report on a mammalian CHO cell-based CRISPR-Cas9 mutag ...[more]