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Dynamic mechanical spectra of salep and casein mixtures.

ABSTRACT: In this study, a glucomannan based salep and casein were mixed in a solution at different concentrations (0-0.75% salep and 0-3.0% casein) according to the central composite rotatable design and their color, steady shear and dynamic shear rheological properties were characterized. All studied parameters were affected by the salep and casein levels significantly. Salep increased the apparent viscosity, consistency coefficient, storage modulus and complex modulus of the solution compared to casein and an interaction was observed between the salep and casein which shows a significant increasing effect on the parameters. Frequency dependency increased with the increase of casein in the mixtures. Regression equations having quite high coefficients of determination were constructed for the estimation of studied parameter levels. Optimization performed with desirability function showed that the maximum levels for the rheological parameters could be achieved by more increment of salep level in the solutions because sole effect of casein on the rheological parameters was limited compared to salep.

SUBMITTER: Karaman S

PROVIDER: S-EPMC6423214 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature

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Dynamic mechanical spectra of salep and casein mixtures.

Karaman Safa S

Journal of food science and technology 20190207 3

In this study, a glucomannan based salep and casein were mixed in a solution at different concentrations (0-0.75% salep and 0-3.0% casein) according to the central composite rotatable design and their color, steady shear and dynamic shear rheological properties were characterized. All studied parameters were affected by the salep and casein levels significantly. Salep increased the apparent viscosity, consistency coefficient, storage modulus and complex modulus of the solution compared to casein ...[more]

PMID: 30956303

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Project description:BackgroundOne-dimensional 1H-NMR spectroscopy is widely used for high-throughput characterization of metabolites in complex biological mixtures. However, the accurate identification of individual compounds is still a challenging task, particularly in spectral regions with higher peak densities. The need for automatic tools to facilitate and further improve the accuracy of such tasks, while using increasingly larger reference spectral libraries becomes a priority of current metabolomics research.ResultsWe introduce a web server application, called MetaboHunter, which can be used for automatic assignment of 1H-NMR spectra of metabolites. MetaboHunter provides methods for automatic metabolite identification based on spectra or peak lists with three different search methods and with possibility for peak drift in a user defined spectral range. The assignment is performed using as reference libraries manually curated data from two major publicly available databases of NMR metabolite standard measurements (HMDB and MMCD). Tests using a variety of synthetic and experimental spectra of single and multi metabolite mixtures show that MetaboHunter is able to identify, in average, more than 80% of detectable metabolites from spectra of synthetic mixtures and more than 50% from spectra corresponding to experimental mixtures. This work also suggests that better scoring functions improve by more than 30% the performance of MetaboHunter's metabolite identification methods.ConclusionsMetaboHunter is a freely accessible, easy to use and user friendly 1H-NMR-based web server application that provides efficient data input and pre-processing, flexible parameter settings, fast and automatic metabolite fingerprinting and results visualization via intuitive plotting and compound peak hit maps. Compared to other published and freely accessible metabolomics tools, MetaboHunter implements three efficient methods to search for metabolites in manually curated data from two reference libraries.

Dataset Information

Dynamic mechanical spectra of salep and casein mixtures.

Publications

Dynamic mechanical spectra of salep and casein mixtures.

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