Dataset Information

Molecular characterisation of the emerging measles virus from Roraima state, Brazil, 2018.

ABSTRACT: Measles is a human infectious disease of global concern that is caused by the measles virus. In this study, we report the complete genome sequencing of one measles virus isolate, genotype D8, that was obtained directly from a urine sample in Boa Vista city, the capital of Roraima state in Brazil. Phylogenetic reconstruction grouped the genome described in this study with that of samples from Australia, South Korea, and Italy. To our knowledge, this is the first complete genome sequence of a wild-type measles virus reported from Latin America. Therefore, the present data strengthen the current knowledge on the molecular epidemiology of measles worldwide.

SUBMITTER: Meneses CAR

PROVIDER: S-EPMC6423661 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature

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Molecular characterisation of the emerging measles virus from Roraima state, Brazil, 2018.

Meneses Cátia Alexandra Ribeiro CAR Nascimento Valdinete Alves do VAD Souza Victor Costa de VC Maito Rodrigo Melo RM Gomes Marconi Aragão MA Cunha Claudeth Rocha Santa Brígida CRSB Antony Ilma de Aguiar IA Silva Maria Eliane Oliveira E MEOE Campos Daniela Palha de Souza DPS Corado André de Lima Guerra ALG Pessoa Karina Pinheiro KP Monteiro Dana Cristina da Silva DCDS Okumoto Osnei O Cunha Marília Coelho MC Pacheco Flávia Caselli FC Naveca Felipe Gomes FG

Memorias do Instituto Oswaldo Cruz 20190318

Measles is a human infectious disease of global concern that is caused by the measles virus. In this study, we report the complete genome sequencing of one measles virus isolate, genotype D8, that was obtained directly from a urine sample in Boa Vista city, the capital of Roraima state in Brazil. Phylogenetic reconstruction grouped the genome described in this study with that of samples from Australia, South Korea, and Italy. To our knowledge, this is the first complete genome sequence of a wild ...[more]

PMID: 30892375

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Project description:National border areas are special places for the spread of Mycobacterium tuberculosis (MTB). These regions concentrate vulnerable populations and constant population movements. Understanding the dynamics of the transmission of MTB is fundamental to propose control measures and to monitor drug resistance. We conducted a population-based prospective study of tuberculosis (TB) to evaluate molecular characteristics of MTB isolates circulating in Roraima, a state on the border of Venezuela and Guyana. Eighty isolates were genotyped by IS6110-RFLP (restriction fragment length polymorphism), spoligotyping, and 24-locus mycobacterial interspersed repetitive unit-variable number of repeats tandem (MIRU-VNTR). Drug susceptibility tests were performed by using the proportion method and GeneXpert® MTB/RIF (Cepheid, Sunnyvale, CA). Isolates showing a phenotypic resistance profile were submitted to polymerase chain reaction (PCR) and sequencing. Spoligotyping showed 40 distinct patterns with a high prevalence of Latin-American and Mediterranean (LAM), Haarlem (H), and the "ill-defined" T clades. Mycobacterial interspersed repetitive unit -VNTR and IS6110-RFLP showed clustering rates of 21.3% and 30%, respectively. Drug resistance was detected in 11 (15.1%) isolates, and all were found to have primary resistance; among these, six (8.2%) isolates were streptomycin mono-resistant, four (5.4%) isoniazid mono-resistant, and one (1.3%) multidrug resistant. This is the first study on the molecular epidemiology and drug resistance profile of MTB from Roraima. Herein, we describe high diversity of genetic profiles circulating in this region that may be driven by the introduction of new strain types because of large population flow in this region. In summary, our results showed that analyses of these circulating strains can contribute to a better understanding of TB epidemiology in the northern Brazilian border and be useful to establish public health policies on TB prevention.

Dataset Information

Molecular characterisation of the emerging measles virus from Roraima state, Brazil, 2018.

Publications

Molecular characterisation of the emerging measles virus from Roraima state, Brazil, 2018.

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