Dataset Information

Global Transcriptional Profiling of the Cyanobacterium Chlorogloeopsis fritschii PCC 9212 in Far-Red Light: Insights Into the Regulation of Chlorophyll d Synthesis.

ABSTRACT: Some terrestrial cyanobacteria can acclimate to and then utilize far-red light (FRL; ? = 700-800 nm) to perform oxygenic photosynthesis through a process called Far-Red Light Photoacclimation (FaRLiP). During FaRLiP, cells synthesize chlorophylls (Chl) d and Chl f and extensively remodel their photosynthetic apparatus by modifying core subunits of photosystem (PS)I, PSII, and the phycobilisome (PBS). Three regulatory proteins, RfpA, RfpB, and RfpC, are encoded in the FaRLiP gene cluster; they sense FRL and control the synthesis of Chl f and expression of the FaRLiP gene cluster. It was previously uncertain if Chl d synthesis and other physiological and metabolic changes to FRL are regulated by RfpABC. In this study we show that Chl d synthesis is regulated by RfpABC; however, most other transcriptional changes leading to the FRL physiological state are not regulated by RfpABC. Surprisingly, we show that erythromycin induces Chl d synthesis in vivo. Transcriptomic and pigment analyses indicate that thiol compounds and/or cysteine proteases could be involved in Chl d synthesis in FRL. We conclude that the protein(s) responsible for Chl d synthesis is/are probably encoded within the FaRLiP gene cluster. Transcriptional responses to FRL help cells to conserve and produce energy and reducing power to overcome implicit light limitation of photosynthesis during the initial acclimation process to FRL.

SUBMITTER: Ho MY

PROVIDER: S-EPMC6424891 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Global Transcriptional Profiling of the Cyanobacterium Chlorogloeopsis fritschii PCC 9212 in Far-Red Light: Insights Into the Regulation of Chlorophyll d Synthesis.

Ho Ming-Yang MY Bryant Donald A DA

Frontiers in microbiology 20190313

Some terrestrial cyanobacteria can acclimate to and then utilize far-red light (FRL; λ = 700-800 nm) to perform oxygenic photosynthesis through a process called Far-Red Light Photoacclimation (FaRLiP). During FaRLiP, cells synthesize chlorophylls (Chl) d and Chl f and extensively remodel their photosynthetic apparatus by modifying core subunits of photosystem (PS)I, PSII, and the phycobilisome (PBS). Three regulatory proteins, RfpA, RfpB, and RfpC, are encoded in the FaRLiP gene cl ...[more]

PMID: 30918500

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Project description:Carotenoids in cyanobacteria play an important role in protecting against and in repairing damage against low level UV-B radiation. Here we use transcriptomics and metabolomic HPLC pigment analysis to compare carotenoid pathway regulation in the filamentous cyanobacterium Chlorogloeopsis fritschii PCC 6912 exposed to white light and to white light supplemented with low level UV-B. Under UV-B changes in carotenoid transcription regulation were found associated with carotenogenesis (carotenoid synthesis), photoprotection and carotenoid cleavage. Transcriptional regulation was reflected in corresponding pigment signatures. All carotenogenesis pathway genes from geranylgeranyl-diphosphate to lycopene were upregulated. There were significant increases in expression of gene homologs (crtW, crtR, cruF, and cruG) associated with routes to ketolation to produce significant increases in echinenone and canthaxanthin concentrations. There were gene homologs for four β-carotene-ketolases (crtO and crtW) present but only one crtW was upregulated. Putative genes encoding enzymes (CruF, CrtR, and CruG) for the conversion of γ-carotene to myxol 2'-methylpentoside were upregulated. The hydroxylation pathway to nostaxanthin via zeaxanthin and caloxanthin (gene homologs for CrtR and CrtG) were not upregulated, reflected in the unchanged corresponding pigment concentrations in zeaxanthin, caloxanthin and nostaxanthin, Transcripts for the non-photochemical quenching related Orange-Carotenoid-Protein (OCP) and associated Fluoresence-Recovery-Protein (FRP) associated with photoprotection were upregulated, and one carotenoid binding Helical-Carotenoid-Protein (HCP) gene homolog was downregulated. Multiple copies of genes encoding putative apocarotenoid related carotenoid oxygenases responsible for carotenoid cleavage were identified, including an upregulated apo-β-carotenal-oxygenase gene homologous to a retinal producing enzyme. Our study provides holistic insight into the photoregulatory processes that modulate the synthesis, photoprotection and cleavage of carotenoids in cyanobacterial cells exposed to low level UV-B. This is important to understanding how regulation of metabolism responds to a changing environment and how metabolism can be modulated for biotechnological purposes.

Dataset Information

Global Transcriptional Profiling of the Cyanobacterium Chlorogloeopsis fritschii PCC 9212 in Far-Red Light: Insights Into the Regulation of Chlorophyll d Synthesis.

Publications

Global Transcriptional Profiling of the Cyanobacterium <i>Chlorogloeopsis fritschii</i> PCC 9212 in Far-Red Light: Insights Into the Regulation of Chlorophyll <i>d</i> Synthesis.

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