Dataset Information

Ultrathin wide-angle large-area digital 3D holographic display using a non-periodic photon sieve.

ABSTRACT: Holographic displays can provide a 3D visual experience to multiple users without requiring special glasses. By precisely tailoring light fields, holographic displays could resemble realistic 3D scenes with full motion parallax and continuous depth cues. However, available holographic displays are unable to generate such scenes given practical limitations in wavefront modulation. In fact, the limited diffraction angle and small number of pixels of current wavefront modulators derive into a 3D scene with small size and narrow viewing angle. We propose a flat-panel wavefront modulator capable of displaying large dynamic holographic images with wide viewing angle. Specifically, an ultrahigh-capacity non-periodic photon sieve, which diffracts light at wide angles, is combined with an off-the-shelf liquid crystal display panel to generate holographic images. Besides wide viewing angle and large screen size, the wavefront modulator provides multi-colour projection and a small form factor, which suggests the possible implementation of holographic displays on thin devices.

SUBMITTER: Park J

PROVIDER: S-EPMC6428928 | biostudies-literature |

REPOSITORIES: biostudies-literature

ACCESS DATA

Similar Datasets

Project description:Patterned two-photon (2P) photolysis via holographic illumination is a powerful method to investigate neuronal function because of its capability to emulate multiple synaptic inputs in three dimensions (3D) simultaneously. However, like any optical system, holographic projectors have a finite space-bandwidth product that restricts the spatial range of patterned illumination or field-of-view (FOV) for a desired resolution. Such trade-off between holographic FOV and resolution restricts the coverage within a limited domain of the neuron's dendritic tree to perform highly resolved patterned 2P photolysis on individual spines. Here, we integrate a holographic projector into a commercial 2P galvanometer-based 2D scanning microscope with an uncaging unit and extend the accessible holographic FOV by using the galvanometer scanning mirrors to reposition the holographic FOV arbitrarily across the imaging FOV. The projector system utilizes the microscope's built-in imaging functions. Stimulation positions can be selected from within an acquired 3D image stack (the volume-of-interest, VOI) and the holographic projector then generates 3D illumination patterns with multiple uncaging foci. The imaging FOV of our system is 800×800 μm2 within which a holographic VOI of 70×70×70 μm3 can be chosen at arbitrary positions and also moved during experiments without moving the sample. We describe the design and alignment protocol as well as the custom software plugin that controls the 3D positioning of stimulation sites. We demonstrate the neurobiological application of the system by simultaneously uncaging glutamate at multiple spines within dendritic domains and consequently observing summation of postsynaptic potentials at the soma, eventually resulting in action potentials. At the same time, it is possible to perform two-photon Ca2+ imaging in 2D in the dendrite and thus to monitor synaptic Ca2+ entry in selected spines and also local regenerative events such as dendritic action potentials.

Dataset Information

Ultrathin wide-angle large-area digital 3D holographic display using a non-periodic photon sieve.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets