Ontology highlight
ABSTRACT:
SUBMITTER: Liu M
PROVIDER: S-EPMC6430498 | biostudies-literature | 2018 Sep
REPOSITORIES: biostudies-literature
ACS chemical biology 20180827 9
APOBEC3B (A3B) deamination activity on ssDNA is considered a contributing factor to tumor heterogeneity and drug resistance in a number of human cancers. Despite its clinical impact, little is known about A3B ssDNA substrate preference. We have used nuclear magnetic resonance to monitor the catalytic turnover of A3B substrates in real-time. This study reports preferred nucleotide sequences for A3B substrates, including optimized 4-mer oligonucleotides, and reveals a breadth of substrate recognit ...[more]