Conformation and Rheological Properties of Calf-Thymus DNA in Solution.
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ABSTRACT: Studies of DNA molecule behavior in aqueous solutions performed through different approaches allow assessment of the solute-solvent interactions and examination of the strong influence of conformation on its physicochemical properties, in the presence of different ionic species and ionic concentrations. Firstly, the conformational behavior of calf-thymus DNA molecules in TE buffer solution is presented as a function of temperature. Secondly, their rheological behavior is discussed, as well as the evidence of the critical concentrations, i.e., the overlap and the entanglement concentrations (C* and Ce, respectively) from steady state flow and oscillatory dynamic shear experiments. The determination of the viscosity in the Newtonian plateau obtained from flow curves ? ( ) allows estimation of the intrinsic viscosity and the specific viscosities at zero shear when C[?] < 40. At end, a generalized master curve is obtained from the variation of the specific viscosity as a function of the overlap parameter C[?]. The variation of the exponent s obtained from the power law ?~ -s for both flow and dynamic results is discussed in terms of Graessley's analysis. In the semi-dilute regime with entanglements, a dynamic master curve is obtained as a function of DNA concentration (CDNA > 2.0 mg/mL) and temperature (10 °C < T < 40 °C).
SUBMITTER: Bravo-Anaya LM
PROVIDER: S-EPMC6432584 | biostudies-literature | 2016 Feb
REPOSITORIES: biostudies-literature
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