Functional assignment of multiple catabolic pathways for D-apiose.
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ABSTRACT:
SUBMITTER: Carter MS
PROVIDER: S-EPMC6435334 | biostudies-literature | 2018 Jul
REPOSITORIES: biostudies-literature
Publications
Functional assignment of multiple catabolic pathways for D-apiose.
Carter Michael S MS Zhang Xinshuai X Huang Hua H Bouvier Jason T JT Francisco Brian San BS Vetting Matthew W MW Al-Obaidi Nawar N Bonanno Jeffrey B JB Ghosh Agnidipta A Zallot Rémi G RG Andersen Harvey M HM Almo Steven C SC Gerlt John A JA
Nature chemical biology 20180604 7
Colocation of the genes encoding ABC, TRAP, and TCT transport systems and catabolic pathways for the transported ligand provides a strategy for discovering novel microbial enzymes and pathways. We screened solute-binding proteins (SBPs) for ABC transport systems and identified three that bind D-apiose, a branched pentose in the cell walls of higher plants. Guided by sequence similarity networks (SSNs) and genome neighborhood networks (GNNs), the identities of the SBPs enabled the discovery of fo ...[more]
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