Ontology highlight
ABSTRACT:
SUBMITTER: McGrath PS
PROVIDER: S-EPMC6436803 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
McGrath Patrick S PS Diette Nicole N Kogut Igor I Bilousova Ganna G
Journal of visualized experiments : JoVE 20181126 141
Induced pluripotent stem cells (iPSCs) have proven to be a valuable tool to study human development and disease. Further advancing iPSCs as a regenerative therapeutic requires a safe, robust, and expedient reprogramming protocol. Here, we present a clinically relevant, step-by-step protocol for the extremely high-efficiency reprogramming of human dermal fibroblasts into iPSCs using a non-integrating approach. The core of the protocol consists of expressing pluripotency factors (SOX2, KLF4, cMYC, ...[more]