Ontology highlight
ABSTRACT:
SUBMITTER: Kogut I
PROVIDER: S-EPMC5821705 | biostudies-literature | 2018 Feb
REPOSITORIES: biostudies-literature
Kogut Igor I McCarthy Sandra M SM Pavlova Maryna M Astling David P DP Chen Xiaomi X Jakimenko Ana A Jones Kenneth L KL Getahun Andrew A Cambier John C JC Pasmooij Anna M G AMG Jonkman Marcel F MF Roop Dennis R DR Bilousova Ganna G
Nature communications 20180221 1
Induced pluripotent stem cells (iPSCs) hold great promise for regenerative medicine; however, their potential clinical application is hampered by the low efficiency of somatic cell reprogramming. Here, we show that the synergistic activity of synthetic modified mRNAs encoding reprogramming factors and miRNA-367/302s delivered as mature miRNA mimics greatly enhances the reprogramming of human primary fibroblasts into iPSCs. This synergistic activity is dependent upon an optimal RNA transfection r ...[more]