Ontology highlight
ABSTRACT:
SUBMITTER: Mao X
PROVIDER: S-EPMC6437208 | biostudies-literature | 2019 Mar
REPOSITORIES: biostudies-literature
Nature communications 20190327 1
The physiological or pathological formation of fibrils often relies on molecular-scale nucleators that finely control the kinetics and structural features. However, mechanistic understanding of how protein nucleators mediate fibril formation in cells remains elusive. Here, we develop a CsgB-decorated DNA origami (CB-origami) to mimic protein nucleators in Escherichia coli biofilm that direct curli polymerization. We show that CB-origami directs curli subunit CsgA monomers to form oligomers and t ...[more]