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NMR resonance assignments of the pathogenesis-related peach allergen Pru p 1.0101.


ABSTRACT: In Europe, Northern America, and China a large number of individuals are suffering from peach (Prunus persica) allergy caused by the protein Pru p 1. Immunologic reactions against this 17.5 kDa protein result from initial sensitization to the birch (Betula verrucosa) pollen allergen Bet v 1 and subsequent immunologic cross-reactivity of Bet v 1 specific antibodies. Allergic symptoms typically include severe itching, scratching of the throat, and rhino conjunctivitis. So far, experimental structural data for the peach allergen Pru p 1 are not available. In a first step towards the elucidation of the structure of this protein we assigned backbone and side chain 1H, 13C, and 15N chemical shifts of the naturally occurring isoform Pru p 1.0101 by solution NMR spectroscopy. Our chemical shift data indicate that this protein fold consists of seven ?-strands separated by two short ?-helices and a long C-terminal ?-helix, which is in accordance with the reported crystal structure of Bet v 1. Our data provide the basis for determining the three-dimensional solution structure of this protein and to characterize its immunologic cross-reactivity on a structural basis.

SUBMITTER: Fuhrer S 

PROVIDER: S-EPMC6439177 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

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NMR resonance assignments of the pathogenesis-related peach allergen Pru p 1.0101.

Führer Sebastian S   Trimmel Simone S   Breuker Kathrin K   Tollinger Martin M  

Biomolecular NMR assignments 20181212 1


In Europe, Northern America, and China a large number of individuals are suffering from peach (Prunus persica) allergy caused by the protein Pru p 1. Immunologic reactions against this 17.5 kDa protein result from initial sensitization to the birch (Betula verrucosa) pollen allergen Bet v 1 and subsequent immunologic cross-reactivity of Bet v 1 specific antibodies. Allergic symptoms typically include severe itching, scratching of the throat, and rhino conjunctivitis. So far, experimental structu  ...[more]

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