Supramolecularly Engineered Circular Bivalent Aptamer for Enhanced Functional Protein Delivery.

ABSTRACT: Circular bivalent aptamers (cb-apt) comprise an emerging class of chemically engineered aptamers with substantially improved stability and molecular recognition ability. Its therapeutic application, however, is challenged by the lack of functional modules to control the interactions of cb-apt with therapeutics. We present the design of a ?-cyclodextrin-modified cb-apt (cb-apt-?CD) and its supramolecular interaction with molecular therapeutics via host-guest chemistry for targeted intracellular delivery. The supramolecular ensemble exhibits high serum stability and enhanced intracellular delivery efficiency compared to a monomeric aptamer. The cb-apt-?CD ensemble delivers green fluorescent protein into targeted cells with efficiency as high as 80%, or cytotoxic saporin to efficiently inhibit tumor cell growth. The strategy of conjugating ?CD to cb-apt, and subsequently modulating the supramolecular chemistry of cb-apt-?CD, provides a general platform to expand and diversify the function of aptamers, enabling new biological and therapeutic applications.

SUBMITTER: Jiang Y 

PROVIDER: S-EPMC6442730 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature