Fragmentation Through Polymerization (FTP): A new method to fragment DNA for next-generation sequencing.
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ABSTRACT:
SUBMITTER: Ignatov KB
PROVIDER: S-EPMC6443234 | biostudies-literature | 2019
REPOSITORIES: biostudies-literature
Publications
Fragmentation Through Polymerization (FTP): A new method to fragment DNA for next-generation sequencing.
Ignatov Konstantin B KB Blagodatskikh Konstantin A KA Shcherbo Dmitry S DS Kramarova Tatiana V TV Monakhova Yulia A YA Kramarov Vladimir M VM
PloS one 20190401 4
Fragmentation of DNA is the very important first step in preparing nucleic acids for next-generation sequencing. Here we report a novel Fragmentation Through Polymerization (FTP) technique, which is a simple, robust, and low-cost enzymatic method of fragmentation. This method generates double-stranded DNA fragments that are suitable for direct use in NGS library construction and allows the elimination of the additional step of reparation of DNA ends. ...[more]
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