SNAC-tag for sequence-specific chemical protein cleavage.
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ABSTRACT:
SUBMITTER: Dang B
PROVIDER: S-EPMC6443254 | biostudies-literature | 2019 Apr
REPOSITORIES: biostudies-literature
Publications
SNAC-tag for sequence-specific chemical protein cleavage.
Dang Bobo B Mravic Marco M Hu Hailin H Schmidt Nathan N Mensa Bruk B DeGrado William F WF
Nature methods 20190325 4
Site-specific protein cleavage is essential for many protein-production protocols and typically requires proteases. We report the development of a chemical protein-cleavage method that is achieved through the use of a sequence-specific nickel-assisted cleavage (SNAC)-tag. We demonstrate that the SNAC-tag can be inserted before both water-soluble and membrane proteins to achieve fusion protein cleavage under biocompatible conditions with efficiency comparable to that of enzymes, and that the meth ...[more]
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