Dataset Information

Identification of long non-coding RNAs expressed in knee and hip osteoarthritic cartilage.

ABSTRACT:

Objective

Long intergenic non-coding RNAs (lincRNAs) are emerging as key regulators in gene expression; however, little is known about the lincRNA expression changes that occur in osteoarthritis (OA). Here we aimed to define a transcriptome of lncRNAs in OA cartilage, specifically comparing the lincRNA transcriptome of knee and hip cartilage.

Method

RNA-seq was performed on nucleic acid extracted from hip cartilage from patients undergoing joint replacement surgery because of either OA (n = 10) or because of a neck of femur fracture (NOF; n = 6). After transcript alignment, counts were performed using Salmon and differential expression for ENSEMBL lincRNAs determined using DESeq2. Hip RNA-seq lincRNA expression was compared to a knee dataset (ArrayExpress; E-MTAB-4304). ChIP-seq data from ENCODE was used to determine whether lincRNAs were associated with promoters (plncRNA) or unidirectional enhancer-like regulatory elements (elncRNAs).

Results

Our analysis of the hip transcriptome identified 1692 expressed Transcripts Per Million (TPM ≥1) Ensembl lincRNAs, of which 198 were significantly (FDR ≤0.05) differentially expressed in OA vs normal (NOF) cartilage. Similar analysis of knee cartilage transcriptome identified 648 Emsembl lincRNAs with 93 significantly (FDR ≤0.05) differentially expressed in intact vs damaged cartilage. In total, 1834 lincRNAs were expressed in both hip and knee cartilage, with a highly significant correlation in expression between the two cartilages.

Conclusion

This is the first study to use RNA-seq to map and compare the lincRNA transcriptomes of hip and knee cartilage. We propose that lincRNAs expressed selectively in cartilage, or showing differential expression in OA, will play a role in cartilage homoeostasis.

SUBMITTER: Ajekigbe B

PROVIDER: S-EPMC6444060 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

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Publications

Identification of long non-coding RNAs expressed in knee and hip osteoarthritic cartilage.

Ajekigbe B B Cheung K K Xu Y Y Skelton A J AJ Panagiotopoulos A A Soul J J Hardingham T E TE Deehan D J DJ Barter M J MJ Young D A DA

Osteoarthritis and cartilage 20190103 4

<h4>Objective</h4>Long intergenic non-coding RNAs (lincRNAs) are emerging as key regulators in gene expression; however, little is known about the lincRNA expression changes that occur in osteoarthritis (OA). Here we aimed to define a transcriptome of lncRNAs in OA cartilage, specifically comparing the lincRNA transcriptome of knee and hip cartilage.<h4>Method</h4>RNA-seq was performed on nucleic acid extracted from hip cartilage from patients undergoing joint replacement surgery because of eith ...[more]

PMID: 30611906

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Dataset Information

Identification of long non-coding RNAs expressed in knee and hip osteoarthritic cartilage.

Objective

Method

Results

Conclusion

Publications

Identification of long non-coding RNAs expressed in knee and hip osteoarthritic cartilage.

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