Ontology highlight
ABSTRACT:
SUBMITTER: Yang J
PROVIDER: S-EPMC6450436 | biostudies-literature | 2019 Apr
REPOSITORIES: biostudies-literature
Yang Jian J Rajan Sandeep S SS Friedrich Mathias J MJ Lan Guocheng G Zou Xiangang X Ponstingl Hannes H Garyfallos Dimitrios A DA Liu Pentao P Bradley Allan A Metzakopian Emmanouil E
Stem cell reports 20190321 4
Primed epiblast stem cells (EpiSCs) can be reverted to a pluripotent embryonic stem cell (ESC)-like state by expression of single reprogramming factor. We used CRISPR activation to perform a genome-scale, reprogramming screen in EpiSCs and identified 142 candidate genes. Our screen validated a total of 50 genes, previously not known to contribute to reprogramming, of which we chose Sall1 for further investigation. We show that Sall1 augments reprogramming of mouse EpiSCs and embryonic fibroblast ...[more]