Dataset Information

Mbt/PAK4 together with SRC modulates N-Cadherin adherens junctions in the developing Drosophila eye.

ABSTRACT: Tissue morphogenesis is accompanied by changes of adherens junctions (AJ). During Drosophila eye development, AJ reorganization includes the formation of isolated N-Cadherin AJ between photoreceptors R3/R4. Little is known about how these N-Cadherin AJ are established and maintained. This study focuses on the kinases Mbt/PAK4 and SRC, both known to alter E-Cadherin AJ across phyla. Drosophila p21-activated kinase Mbt and the non-receptor tyrosine kinases Src64 and Src42 regulate proper N-Cadherin AJ. N-Cadherin AJ elongation depends on SRC kinase activity. Cell culture experiments demonstrate binding of both Drosophila SRC isoforms to N-Cadherin and its subsequent tyrosine phosphorylation. In contrast, Mbt stabilizes but does not bind N-Cadherin in vitro Mbt is required in R3/R4 for zipping the N-Cadherin AJ between these cells, independent of its kinase activity and Cdc42-binding. The mbt phenotype can be reverted by mutations in Src64 and Src42 Because Mbt neither directly binds to SRC proteins nor has a reproducible influence on their kinase activity, the conclusion is that Mbt and SRC signaling converge on N-Cadherin. N-Cadherin AJ formation during eye development requires a proper balance between the promoting effects of Mbt and the inhibiting influences of SRC kinases.

SUBMITTER: Putz SM

PROVIDER: S-EPMC6451336 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature

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Mbt/PAK4 together with SRC modulates N-Cadherin adherens junctions in the developing Drosophila eye.

Pütz Stephanie M SM

Biology open 20190318 3

Tissue morphogenesis is accompanied by changes of adherens junctions (AJ). During Drosophila eye development, AJ reorganization includes the formation of isolated N-Cadherin AJ between photoreceptors R3/R4. Little is known about how these N-Cadherin AJ are established and maintained. This study focuses on the kinases Mbt/PAK4 and SRC, both known to alter E-Cadherin AJ across phyla. Drosophila p21-activated kinase Mbt and the non-receptor tyrosine kinases Src64 and Src42 regulate pr ...[more]

PMID: 30885947

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Dataset Information

Mbt/PAK4 together with SRC modulates N-Cadherin adherens junctions in the developing Drosophila eye.

Publications

Mbt/PAK4 together with SRC modulates N-Cadherin adherens junctions in the developing <i>Drosophila</i> eye.

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