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RNase I regulates Escherichia coli 2',3'-cyclic nucleotide monophosphate levels and biofilm formation.


ABSTRACT: Regulation of nucleotide and nucleoside concentrations is critical for faithful DNA replication, transcription, and translation in all organisms, and has been linked to bacterial biofilm formation. Unusual 2',3'-cyclic nucleotide monophosphates (2',3'-cNMPs) recently were quantified in mammalian systems, and previous reports have linked these nucleotides to cellular stress and damage in eukaryotes, suggesting an intriguing connection with nucleotide/nucleoside pools and/or cyclic nucleotide signaling. This work reports the first quantification of 2',3'-cNMPs in Escherichia coli and demonstrates that 2',3'-cNMP levels in E. coli are generated specifically from RNase I-catalyzed RNA degradation, presumably as part of a previously unidentified nucleotide salvage pathway. Furthermore, RNase I and 2',3'-cNMP levels are demonstrated to play an important role in controlling biofilm formation. This work identifies a physiological role for cytoplasmic RNase I and constitutes the first progress toward elucidating the biological functions of bacterial 2',3'-cNMPs.

SUBMITTER: Fontaine BM 

PROVIDER: S-EPMC6452634 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature

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RNase I regulates <i>Escherichia coli</i> 2',3'-cyclic nucleotide monophosphate levels and biofilm formation.

Fontaine Benjamin M BM   Martin Kevin S KS   Garcia-Rodriguez Jennifer M JM   Jung Claire C   Briggs Laura L   Southwell Jessica E JE   Jia Xin X   Weinert Emily E EE  

The Biochemical journal 20180430 8


Regulation of nucleotide and nucleoside concentrations is critical for faithful DNA replication, transcription, and translation in all organisms, and has been linked to bacterial biofilm formation. Unusual 2',3'-cyclic nucleotide monophosphates (2',3'-cNMPs) recently were quantified in mammalian systems, and previous reports have linked these nucleotides to cellular stress and damage in eukaryotes, suggesting an intriguing connection with nucleotide/nucleoside pools and/or cyclic nucleotide sign  ...[more]

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