Live Cell Analysis of Shear Stress on Pseudomonas aeruginosa Using an Automated Higher-Throughput Microfluidic System.
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ABSTRACT:
SUBMITTER: Sutlief AL
PROVIDER: S-EPMC6455916 | biostudies-literature | 2019 Jan
REPOSITORIES: biostudies-literature
Publications
Live Cell Analysis of Shear Stress on Pseudomonas aeruginosa Using an Automated Higher-Throughput Microfluidic System.
Sutlief Arin L AL Valquier-Flynn Helena H Wilson Christina C Perez Marco M Kleinschmidt Hunter H Schofield Brett J BJ Delmain Elizabeth E Holmes Andrea E AE Wentworth Christopher D CD
Journal of visualized experiments : JoVE 20190116 143
A higher-throughput microfluidic in vitro bioreactor coupled with fluorescence microscopy has been used to study bacterial biofilm growth and morphology, including Pseudomonas aeruginosa (P. aeruginosa). Here, we will describe how the system can be used to study the growth kinetics and the morphological properties such as the surface roughness and textural entropy of P. aeruginosa strain PA01 that expresses an enhanced green fluorescent protein (PA01-EGFP). A detailed protocol will describe how ...[more]
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