Ontology highlight
ABSTRACT:
SUBMITTER: Abdeladim L
PROVIDER: S-EPMC6458155 | biostudies-literature | 2019 Apr
REPOSITORIES: biostudies-literature
Abdeladim Lamiae L Matho Katherine S KS Clavreul Solène S Mahou Pierre P Sintes Jean-Marc JM Solinas Xavier X Arganda-Carreras Ignacio I Turney Stephen G SG Lichtman Jeff W JW Chessel Anatole A Bemelmans Alexis-Pierre AP Loulier Karine K Supatto Willy W Livet Jean J Beaurepaire Emmanuel E
Nature communications 20190410 1
Large-scale microscopy approaches are transforming brain imaging, but currently lack efficient multicolor contrast modalities. We introduce chromatic multiphoton serial (ChroMS) microscopy, a method integrating one-shot multicolor multiphoton excitation through wavelength mixing and serial block-face image acquisition. This approach provides organ-scale micrometric imaging of spectrally distinct fluorescent proteins and label-free nonlinear signals with constant micrometer-scale resolution and s ...[more]