Ontology highlight
ABSTRACT:
SUBMITTER: Yang R
PROVIDER: S-EPMC5517475 | biostudies-literature | 2017 Jul
REPOSITORIES: biostudies-literature
Yang Ruohui R Weber Timothy D TD Witkowski Ellen D ED Davison Ian G IG Mertz Jerome J
Scientific reports 20170719 1
Multiphoton microscopes are hampered by limited dynamic range, preventing weak sample features from being detected in the presence of strong features, or preventing the capture of unpredictable bursts in sample strength. We present a digital electronic add-on technique that vastly improves the dynamic range of a multiphoton microscope while limiting potential photodamage. The add-on provides real-time negative feedback to regulate the laser power delivered to the sample, and a log representation ...[more]