Dataset Information

Temperature-dependent seminal recovery in the southern king crab Lithodes santolla.

ABSTRACT: Male-biased fishery management can provoke depletion of seminal reserves, which is the primary cause of sperm limitation. Therefore, identifying factors which contribute to the vulnerability to depletion of seminal reserves is a priority. The present study aimed to determine the effect of temperature on the recovery rate of sperm and seminal reserves after their depletion in Lithodes santolla, an important fishery resource in southern Chile. Sperm and seminal reserves were not fully recovered within 30 days. Temperature significantly affected seminal recovery: after 30 days the recovery index increased to 40% and 21% at 9°C and 12°C, respectively. The twice as fast seminal recovery at 9°C may be explained by the zone of origin of the individuals in this study (northern distributional limit), and 12°C may be close to the threshold of temperature tolerance. Lithodes santolla populations subject to intense male-only fisheries may be vulnerable to depletion of seminal reserves and a climate change scenario could additionally aggravate the risk of seminal depletion in L. santolla in its northern distributional limit.

SUBMITTER: Pretterebner K

PROVIDER: S-EPMC6458361 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature

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Temperature-dependent seminal recovery in the southern king crab <i>Lithodes santolla</i>.

Pretterebner Katrin K Pardo Luis Miguel LM Paschke Kurt K

Royal Society open science 20190306 3

Male-biased fishery management can provoke depletion of seminal reserves, which is the primary cause of sperm limitation. Therefore, identifying factors which contribute to the vulnerability to depletion of seminal reserves is a priority. The present study aimed to determine the effect of temperature on the recovery rate of sperm and seminal reserves after their depletion in <i>Lithodes santolla</i>, an important fishery resource in southern Chile. Sperm and seminal reserves were not fully recov ...[more]

PMID: 31032024

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Project description:The purpose of this study was to test the hypothesis that the genetic diversity of commercially significant species of King Crabs (Lithodes spp.) along the south-eastern Pacific (SEP) comprises different independent evolutionary units (IEUs) with spatially isolated distribution. Nine localities from inner and open waters along the SEP Chilean coast (39°S-55°S) were sampled. We analyzed sequences from 173 individuals for the mitochondrial gene Cytochrome oxidase I (COX-I), 151 individuals for the Internal Transcribed Spacer 1 (ITS) and 135 for the structural ribosomal RNA (28S). Genetic delimitation was performed through three analytical methods: ABGD, GMYC, and its Bayesian implementation, bGMYC. Bayesian phylogenetic analyses and haplotype networks were also performed. Divergence time between clades was assessed for the COX-I marker and estimated from known evolutionary rates for this marker in other crustacean species and fossil calibration from other Anomuran species. Delimitation analyses, phylogenetic analyses, and mitochondrial haplotype networks suggested the presence of two deeply divergent mitochondrial lineages of Lithodes in the SEP, referred to as Clade1 and Clade 2. Nuclear markers showed low phylogenetic resolution and therefore were unsuitable for molecular species delimitation. Divergence time analysis of the mitochondrial lineages suggests a separation between Clades of approximately 2.3 Mya. The divergence time obtained suggested that Pliocene glaciations and deglaciations cycles could be involved in hybridization events between Lithodes IEUs at southern tip of South American coasts. The different frequencies of Lithodes haplotypes in inner and open water environments along SEP coasts could be explained by events such as the last glacial maximum or by differences in the adaptation of each clade to different environments. These findings support the necessity of evaluating the taxonomic status of Lithodes individuals found along SEP coasts under an integrative taxonomy approach or through markers with other evolution rates than those already used.

Project description:1. The properties of fructose diphosphatase from skeletal muscle of the Alaskan king-crab (Paralithodes camtschatica) were examined over the physiological temperature range of the animal. 2. King-crab muscle fructose diphosphatase is first activated by Na(+) and NH(4) (+) and is then partially inhibited by these cations at concentrations higher than 10mm at 0 degrees , 8 degrees and 15 degrees C. Enzyme activity is stimulated by K(+) at 0 degrees C, but is curtailed at 8 degrees C and 15 degrees C, an effect that could render rate independent of temperature. 3. Affinity for substrate increases with decreasing temperature; below the temperature of acclimatization, K(m) for fructose 1,6-diphosphate increases, resulting in a complex U-shaped temperature-K(m) curve. 4. King-crab muscle fructose diphosphatase is inhibited by low concentrations of AMP. As with enzymes of other poikilotherms, inhibition by AMP is sensitive to temperature; the enzyme is least sensitive to inhibition by AMP near the temperature of acclimatization. 5. The affinity of fructose diphosphatase for fructose 1,6-diphosphate is enhanced by phosphoenolpyruvate, and this activation is temperature-sensitive; 0.5mm-phosphoenolpyruvate causes a sevenfold decrease in K(m) for fructose 1,6-diphosphate at 15 degrees C but a 25-fold decrease at 0 degrees C. 6. Phosphoenolpyruvate appears to decrease the affinity of king-crab muscle fructose diphosphatase for AMP at low temperature, whereas at the higher temperature it appears to enhance inhibition by AMP. Phosphoenolpyruvate was not observed to cause a reversal of inhibition of fructose diphosphatase activity by AMP. The identification of phosphoenolpyruvate as an activator of a rate-limiting step in gluconeogenesis permits the suggestion of a coupling of the controlling mechanisms of several steps in the glycolytic and gluconeogenic chains. 7. These findings suggest mechanisms for the maintenance and regulation of control of fructose diphosphatase activity in king-crab skeletal muscle at low temperature and under conditions that favour concomitant activity of phosphofructokinase.

Dataset Information

Temperature-dependent seminal recovery in the southern king crab Lithodes santolla.

Publications

Temperature-dependent seminal recovery in the southern king crab <i>Lithodes santolla</i>.

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