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Plug-and-play metabolic transducers expand the chemical detection space of cell-free biosensors.

ABSTRACT: Cell-free transcription-translation systems have great potential for biosensing, yet the range of detectable chemicals is limited. Here we provide a workflow to expand the range of molecules detectable by cell-free biosensors through combining synthetic metabolic cascades with transcription factor-based networks. These hybrid cell-free biosensors have a fast response time, strong signal response, and a high dynamic range. In addition, they are capable of functioning in a variety of complex media, including commercial beverages and human urine, in which they can be used to detect clinically relevant concentrations of small molecules. This work provides a foundation to engineer modular cell-free biosensors tailored for many applications.

SUBMITTER: Voyvodic PL

PROVIDER: S-EPMC6461607 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

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Plug-and-play metabolic transducers expand the chemical detection space of cell-free biosensors.

Voyvodic Peter L PL Pandi Amir A Koch Mathilde M Conejero Ismael I Valjent Emmanuel E Courtet Philippe P Renard Eric E Faulon Jean-Loup JL Bonnet Jerome J

Nature communications 20190412 1

Cell-free transcription-translation systems have great potential for biosensing, yet the range of detectable chemicals is limited. Here we provide a workflow to expand the range of molecules detectable by cell-free biosensors through combining synthetic metabolic cascades with transcription factor-based networks. These hybrid cell-free biosensors have a fast response time, strong signal response, and a high dynamic range. In addition, they are capable of functioning in a variety of complex media ...[more]

PMID: 30979906

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