Ontology highlight
ABSTRACT:
SUBMITTER: Jung JK
PROVIDER: S-EPMC7718425 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Nature biotechnology 20200706 12
Lack of access to safe drinking water is a global problem, and methods to reliably and easily detect contaminants could be transformative. We report the development of a cell-free in vitro transcription system that uses RNA Output Sensors Activated by Ligand Induction (ROSALIND) to detect contaminants in water. A combination of highly processive RNA polymerases, allosteric protein transcription factors and synthetic DNA transcription templates regulates the synthesis of a fluorescence-activating ...[more]