Ontology highlight
ABSTRACT:
SUBMITTER: Liu N
PROVIDER: S-EPMC6464196 | biostudies-literature | 2019 Apr
REPOSITORIES: biostudies-literature
Liu Na N Jiang Shijun S Feng Songli S Shang Wenyan W Xing Guozhen G Qiu Rui R Li Chengjun C Li Shujun S Zheng Wenming W
The plant pathology journal 20190401 2
A duplex PCR method was developed for simultaneous detection and identification of tobacco root rot pathogens <i>Phytophthora nicotianae</i> and <i>Thielaviopsis basicola</i>. The specific primers for <i>P. nicotianae</i> were developed based on its internal transcribed spacer (ITS) regions of ribosomal gene, <i>ras</i> gene and <i>hgd</i> gene, while the specific primers for <i>T. basicola</i> were designed based on its ITS regions and <i>β-</i>tubulin gene. The specificity of the primers was d ...[more]