Unknown

Dataset Information

0

Mass Spectrometry-based Label-free Quantitative Proteomics To Study the Effect of 3PO Drug at Cellular Level.


ABSTRACT: Human endothelial cells (ECs) have been employed to monitor the protein changes induced by [3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one] (3PO), a compound able to inhibit the glycolytic flux partially and transiently and to reduce pathological angiogenesis in a variety of disease models. Normal and TNF? induced inflamed ECs were incubated with and without 3PO at a concentration (20 ?M) able to inhibit cell proliferation without cell death. At the end of the incubation period, samples were submitted to the following steps: (a) whole protein extraction, reduction, alkylation, and digestion by trypsin; (b) peptide separation by nano-LC-MS/MS analysis using a high-resolution mass spectrometer; (c) data analysis including protein identification, quantification, and statistical analysis. An altered protein expression profiling in combination with protein network analysis was employed by using a mass spectrometry-based label-free quantification approach to explore the underlying mechanisms of 3PO at cellular level.

SUBMITTER: Nukala SB 

PROVIDER: S-EPMC6466823 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC4148364 | biostudies-literature
| S-EPMC3592467 | biostudies-literature
| S-EPMC11208500 | biostudies-literature
| S-EPMC4587597 | biostudies-literature
| S-EPMC5814520 | biostudies-literature
| S-EPMC6249692 | biostudies-literature
| S-EPMC5618239 | biostudies-literature
| S-EPMC2667347 | biostudies-literature
| S-EPMC7642908 | biostudies-literature
| S-EPMC1992440 | biostudies-literature