Metagenomic engineering of the mammalian gut microbiome in situ.
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ABSTRACT:
SUBMITTER: Ronda C
PROVIDER: S-EPMC6467691 | biostudies-literature | 2019 Feb
REPOSITORIES: biostudies-literature
Publications
Metagenomic engineering of the mammalian gut microbiome in situ.
Ronda Carlotta C Chen Sway P SP Cabral Vitor V Yaung Stephanie J SJ Wang Harris H HH
Nature methods 20190114 2
Engineering of microbial communities in open environments remains challenging. Here we describe a platform used to identify and modify genetically tractable mammalian microbiota by engineering community-wide horizontal gene transfer events in situ. With this approach, we demonstrate that diverse taxa in the mouse gut microbiome can be modified directly with a desired genetic payload. In situ microbiome engineering in living animals allows novel capabilities to be introduced into established comm ...[more]
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