Reproducibility of fluid-phase measurements in PBS-treated sputum supernatant of healthy and stable COPD subjects.

ABSTRACT:

Purpose

The purpose of this study was to investigate the reproducibility of fluid-phase measurements in PBS-treated sputum supernatant, processed using the two-step method, of healthy and stable COPD individuals.

Methods

Nine healthy subjects and 23 stable COPD patients provided sputum twice within 6 days. A two-step sputum processing method was used to obtain PBS-treated supernatant and sputum cells. Soluble protein markers and IgG and IgM autoantibody profiles in PBS supernatant were analyzed using customized microarrays. Repeatability of measurements was assessed by paired-sample testing and an intraclass correlation coefficient, then graphically reported by Bland-Altman plot.

Results

There was no significant difference between the repeated detection of 8/10 types

SUBMITTER: Wang F 

PROVIDER: S-EPMC6469484 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature