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Cohesin-Mediated Genome Architecture Does Not Define DNA Replication Timing Domains.

ABSTRACT: 3D genome organization is strongly predictive of DNA replication timing in mammalian cells. This work tested the extent to which loop-based genome architecture acts as a regulatory unit of replication timing by using an auxin-inducible system for acute cohesin ablation. Cohesin ablation in a population of cells in asynchronous culture was shown not to disrupt patterns of replication timing as assayed by replication sequencing (RepliSeq) or BrdU-focus microscopy. Furthermore, cohesin ablation prior to S phase entry in synchronized cells was similarly shown to not impact replication timing patterns. These results suggest that cohesin-mediated genome architecture is not required for the execution of replication timing patterns in S phase, nor for the establishment of replication timing domains in G1.

SUBMITTER: Oldach P

PROVIDER: S-EPMC6471042 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature

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Cohesin-Mediated Genome Architecture Does Not Define DNA Replication Timing Domains.

Oldach Phoebe P Nieduszynski Conrad A CA

Genes 20190304 3

3D genome organization is strongly predictive of DNA replication timing in mammalian cells. This work tested the extent to which loop-based genome architecture acts as a regulatory unit of replication timing by using an auxin-inducible system for acute cohesin ablation. Cohesin ablation in a population of cells in asynchronous culture was shown not to disrupt patterns of replication timing as assayed by replication sequencing (RepliSeq) or BrdU-focus microscopy. Furthermore, cohesin ablation pri ...[more]

PMID: 30836708

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