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Accelerated CO? Hydration with Thermostable Sulfurihydrogenibium azorense Carbonic Anhydrase-Chitin Binding Domain Fusion Protein Immobilised on Chitin Support.


ABSTRACT: Carbonic anhydrases (CAs) represent a group of enzymes that catalyse important reactions of carbon dioxide hydration and dehydration, a reaction crucial to many biological processes and environmental biotechnology. In this study we successfully constructed a thermostable fusion enzyme composed of the Sulfurihydrogenibium azorense carbonic anhydrase (Saz_CA), the fastest CA discovered to date, and the chitin binding domain (ChBD) of chitinase from Bacillus circulans. Introduction of ChBD to the Saz_CA had no major impact on the effect of ions or inhibitors on the enzymatic activity. The fusion protein exhibited no negative effects up to 60 °C, whilst the fusion partner appears to protect the enzyme from negative effects of magnesium. The prepared biocatalyst appears to be thermally activated at 60 °C and could be partially purified with heat treatment. Immobilisation attempts on different kinds of chitin-based support results have shown that the fusion enzyme preferentially binds to a cheap, untreated chitin with a large crystallinity index over more processed forms of chitin. It suggests significant potential economic benefits for large-scale deployment of immobilised CA technologies such as CO? utilisation or mineralisation.

SUBMITTER: Hou J 

PROVIDER: S-EPMC6471549 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature

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Accelerated CO₂ Hydration with Thermostable <i>Sulfurihydrogenibium azorense</i> Carbonic Anhydrase-Chitin Binding Domain Fusion Protein Immobilised on Chitin Support.

Hou Juan J   Li Xingkang X   Kaczmarek Michal B MB   Chen Pengyu P   Li Kai K   Jin Peng P   Liang Yuanmei Y   Daroch Maurycy M  

International journal of molecular sciences 20190325 6


Carbonic anhydrases (CAs) represent a group of enzymes that catalyse important reactions of carbon dioxide hydration and dehydration, a reaction crucial to many biological processes and environmental biotechnology. In this study we successfully constructed a thermostable fusion enzyme composed of the <i>Sulfurihydrogenibium azorense</i> carbonic anhydrase (Saz_CA), the fastest CA discovered to date, and the chitin binding domain (ChBD) of chitinase from <i>Bacillus circulans</i>. Introduction of  ...[more]

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