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Influence of high glucose on mesangial cell-derived exosome composition, secretion and cell communication.

ABSTRACT: Mesangial cells stimulated with high glucose (HG) exhibit increased intracellular angiotensin II (AngII) synthesis that is correlated with the upregulation of AngII target genes, such as profibrotic cytokines. The intracrine effects of AngII can be mediated by several molecules transferred to other cells via exosomes (Exos), which play a key role in cellular communication under many physiological and pathological conditions. The aim of this study was to investigate the effects of exosomes derived from HG-stimulated human mesangial cells (HG-HMCs) on normal unstimulated HMCs. Exosomes from HMCs (C-Exos) and HG-HMCs (HG-Exos) were obtained from cell culture supernatants. HMCs were incubated with C-Exos or HG-Exos. HG stimulus induced a change in the amount but not the size of Exos. Both C-Exos and HG-Exos contained angiotensinogen and renin, but no angiotensin converting enzyme was detected. Compared with HMCs treated with C-Exos, HMCs treated with HG-Exos presented higher levels of fibronectin, angiotensinogen, renin, AT₁ and AT₂ receptors, indicating that HG-Exos modified the function of normal HMCs. These results suggest that the intercellular communication through Exos may have pathophysiological implications in the diabetic kidney.

SUBMITTER: da Silva Novaes A

PROVIDER: S-EPMC6472340 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

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Influence of high glucose on mesangial cell-derived exosome composition, secretion and cell communication.

da Silva Novaes Antônio A Borges Fernanda Teixeira FT Maquigussa Edgar E Varela Vanessa Araújo VA Dias Marcos Vinicios Salles MVS Boim Mirian Aparecida MA

Scientific reports 20190418 1

Mesangial cells stimulated with high glucose (HG) exhibit increased intracellular angiotensin II (AngII) synthesis that is correlated with the upregulation of AngII target genes, such as profibrotic cytokines. The intracrine effects of AngII can be mediated by several molecules transferred to other cells via exosomes (Exos), which play a key role in cellular communication under many physiological and pathological conditions. The aim of this study was to investigate the effects of exosomes derive ...[more]

PMID: 31000742

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Influence of high glucose on mesangial cell-derived exosome composition, secretion and cell communication.

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Influence of high glucose on mesangial cell-derived exosome composition, secretion and cell communication.

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