Ontology highlight
ABSTRACT:
SUBMITTER: Harnos J
PROVIDER: S-EPMC6472409 | biostudies-literature | 2019 Apr
REPOSITORIES: biostudies-literature
Nature communications 20190418 1
Dishevelled (DVL) is the key component of the Wnt signaling pathway. Currently, DVL conformational dynamics under native conditions is unknown. To overcome this limitation, we develop the Fluorescein Arsenical Hairpin Binder- (FlAsH-) based FRET in vivo approach to study DVL conformation in living cells. Using this single-cell FRET approach, we demonstrate that (i) Wnt ligands induce open DVL conformation, (ii) DVL variants that are predominantly open, show more even subcellular localization and ...[more]