Ontology highlight
ABSTRACT:
SUBMITTER: Harnos J
PROVIDER: S-EPMC6472409 | biostudies-literature | 2019 Apr
REPOSITORIES: biostudies-literature
Harnoš Jakub J Cañizal Maria Consuelo Alonso MCA Jurásek Miroslav M Kumar Jitender J Holler Cornelia C Schambony Alexandra A Hanáková Kateřina K Bernatík Ondřej O Zdráhal Zbyněk Z Gömöryová Kristína K Gybeľ Tomáš T Radaszkiewicz Tomasz Witold TW Kravec Marek M Trantírek Lukáš L Ryneš Jan J Dave Zankruti Z Fernández-Llamazares Ana Iris AI Vácha Robert R Tripsianes Konstantinos K Hoffmann Carsten C Bryja Vítězslav V
Nature communications 20190418 1
Dishevelled (DVL) is the key component of the Wnt signaling pathway. Currently, DVL conformational dynamics under native conditions is unknown. To overcome this limitation, we develop the Fluorescein Arsenical Hairpin Binder- (FlAsH-) based FRET in vivo approach to study DVL conformation in living cells. Using this single-cell FRET approach, we demonstrate that (i) Wnt ligands induce open DVL conformation, (ii) DVL variants that are predominantly open, show more even subcellular localization and ...[more]