Ontology highlight
ABSTRACT:
SUBMITTER: Sparks JL
PROVIDER: S-EPMC6475077 | biostudies-literature | 2019 Jan
REPOSITORIES: biostudies-literature
Cell 20181227 1-2
Covalent DNA-protein cross-links (DPCs) impede replication fork progression and threaten genome integrity. Using Xenopus egg extracts, we previously showed that replication fork collision with DPCs causes their proteolysis, followed by translesion DNA synthesis. We show here that when DPC proteolysis is blocked, the replicative DNA helicase CMG (CDC45, MCM2-7, GINS), which travels on the leading strand template, bypasses an intact leading strand DPC. Single-molecule imaging reveals that GINS doe ...[more]