Unknown

Dataset Information

0

MiR-31-5p Is a LIPUS-Mechanosensitive MicroRNA that Targets HIF-1? Signaling and Cytoskeletal Proteins.


ABSTRACT: The roles of low-intensity pulsed ultrasound (LIPUS) and microRNAs (miRNAs) on hMSCs commitments have already been investigated; however, the effects of the application of their co-treatments in an in vitro cell model are still unknown. Our previous studies demonstrated that (i) LIPUS modulated hMSCs cytoskeletal organization and (ii) miRNA-675-5p have a role in HIF-1? signaling modulation during hMSCs osteoblast commitment. We investigated for the first time the role of LIPUS as promoter tool for miRNA expression. Thanks to bioinformatic analysis, we identified miR-31-5p as a LIPUS-induced miRNA and investigated its role through in vitro studies of gain and loss of function. Results highlighted that LIPUS stimulation induced a hypoxia adaptive cell response, which determines a reorganization of cell membrane and cytoskeleton proteins. MiR-31-5p gain and loss of function studies, demonstrated as miR-31-5p overexpression, were able to induce hypoxic and cytoskeletal responses. Moreover, the co-treatments LIPUS and miR-31-5p inhibitor abolished the hypoxic responses including angiogenesis and the expression of Rho family proteins. MiR-31-5p was identified as a LIPUS-mechanosensitive miRNAs and may be considered a new therapeutic option to promote or abolish hypoxic response and cytoskeletal organization on hMSCs during the bone regeneration process.

SUBMITTER: Costa V 

PROVIDER: S-EPMC6480017 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications

miR-31-5p Is a LIPUS-Mechanosensitive MicroRNA that Targets HIF-1α Signaling and Cytoskeletal Proteins.

Costa Viviana V   Carina Valeria V   Conigliaro Alice A   Raimondi Lavinia L   De Luca Angela A   Bellavia Daniele D   Salamanna Francesca F   Setti Stefania S   Alessandro Riccardo R   Fini Milena M   Giavaresi Gianluca G  

International journal of molecular sciences 20190328 7


The roles of low-intensity pulsed ultrasound (LIPUS) and microRNAs (miRNAs) on hMSCs commitments have already been investigated; however, the effects of the application of their co-treatments in an in vitro cell model are still unknown. Our previous studies demonstrated that (i) LIPUS modulated hMSCs cytoskeletal organization and (ii) miRNA-675-5p have a role in HIF-1α signaling modulation during hMSCs osteoblast commitment. We investigated for the first time the role of LIPUS as promoter tool f  ...[more]

Similar Datasets

| 2260465 | ecrin-mdr-crc
| S-EPMC7272402 | biostudies-literature
| S-EPMC8766956 | biostudies-literature
| S-EPMC8406720 | biostudies-literature
| S-EPMC8941519 | biostudies-literature
2022-02-26 | E-MTAB-11160 | biostudies-arrayexpress
| S-EPMC6584523 | biostudies-literature
| S-EPMC6739051 | biostudies-literature
| S-EPMC6822233 | biostudies-literature
| S-EPMC8427313 | biostudies-literature