Ontology highlight
ABSTRACT:
SUBMITTER: Sosson M
PROVIDER: S-EPMC6486630 | biostudies-literature | 2019 May
REPOSITORIES: biostudies-literature
Sosson Marilyne M Pfeffer Daniel D Richert Clemens C
Nucleic acids research 20190501 8
The template-directed formation of phosphodiester bonds between two nucleic acid components is a pivotal process in biology. To induce such a reaction in the absence of enzymes is a challenge. This challenge has been met for the extension of a primer with mononucleotides, but the ligation of short oligonucleotides (dimers or trimers) has proven difficult. Here we report a method for ligating dimers and trimers of ribonucleotides using in situ activation in aqueous buffer. All 16 different dimers ...[more]