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Genomic characterization of hepatitis C virus transmitted founder variants with deep sequencing.


ABSTRACT: Transfer of hepatitis C virus (HCV) infection from a donor to a new recipient is associated with a bottleneck of genetic diversity in the transmitted viral variants. Existing data suggests that one, or very few, variants emerge from this bottleneck to establish the infection (transmitted founder [T/F] variants). In HCV, very few T/F variants have been characterized due to the challenges of obtaining early infection samples and of high throughput viral genome sequencing. This study used a large, acute HCV, deep-sequenced dataset from first viremia samples collected in nine prospective cohorts across four countries, to estimate the prevalence of single T/F viruses, and to identify host and virus-related factors associated with infections initiated by a single T/F variant. The short reads generated by Illumina sequencing were used to reconstruct viral haplotypes with two haplotype reconstruction algorithms. The haplotypes were examined for random mutations (Poisson distribution) and a star-like phylogeny to identify T/F viruses. The findings were cross-validated by haplotype reconstructions across three regions of the genome (Core-E2, NS3, NS5A) to minimize the possibility of spurious overestimation of single T/F variants. Of 190 acute infection samples examined, 54 were very early acute infections (HCV antibody negative, RNA positive), and single transmitted founders were identified in 14 (26%, 95% CI: 16-39%) after cross validation across multiple regions of the genome with two haplotype reconstruction algorithms. The presence of a single T/F virus was not associated with any host or virus-related factors, notably viral genotype or spontaneous clearance. In conclusion, approximately one in four new HCV infections originates from a single T/F virus. Resolution of genomic sequences of single T/F variants is the first step in exploring unique properties of these variants in the infection of host hepatocytes.

SUBMITTER: Abayasingam A 

PROVIDER: S-EPMC6487228 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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Genomic characterization of hepatitis C virus transmitted founder variants with deep sequencing.

Abayasingam Arunasingam A   Leung Preston P   Eltahla Auda A   Bull Rowena A RA   Luciani Fabio F   Grebely Jason J   Dore Gregory J GJ   Applegate Tanya T   Page Kimberly K   Bruneau Julie J   Cox Andrea L AL   Kim Arthur Y AY   Schinkel Janke J   Shoukry Naglaa H NH   Lauer Georg M GM   Maher Lisa L   Hellard Margaret M   Prins Maria M   Lloyd Andrew A   Rodrigo Chaturaka C  

Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases 20190308


Transfer of hepatitis C virus (HCV) infection from a donor to a new recipient is associated with a bottleneck of genetic diversity in the transmitted viral variants. Existing data suggests that one, or very few, variants emerge from this bottleneck to establish the infection (transmitted founder [T/F] variants). In HCV, very few T/F variants have been characterized due to the challenges of obtaining early infection samples and of high throughput viral genome sequencing. This study used a large,  ...[more]

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