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Development of an anti-bear podoplanin monoclonal antibody PMab-247 for immunohistochemical analysis.

ABSTRACT: Sensitive and specific monoclonal antibodies (mAbs) targeting podoplanin (PDPN) are needed for immunohistochemical analyses using formalin-fixed paraffin-embedded tissues because PDPN is known as a lymphatic endothelial cell maker in pathology. Recently, we established anti-PDPN mAbs against many species, such as human, mouse, rat, rabbit, dog, cat, bovine, pig, horse, goat, tiger, alpaca, and Tasmanian devil. However, anti-bear PDPN (bPDPN) has not been established yet. In this study, we immunized mice with bPDPN-overexpressing Chinese hamster ovary (CHO)-K1 (CHO/bPDPN) cells, and screened mAbs against bPDPN using flow cytometry. One of the mAbs, PMab-247 (IgG1, kappa), specifically detected CHO/bPDPN cells by flow cytometry and immunohistochemistry. Our findings suggest the potential usefulness of PMab-247 for the functional analyses of bPDPN.

SUBMITTER: Furusawa Y

PROVIDER: S-EPMC6488525 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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Development of an anti-bear podoplanin monoclonal antibody PMab-247 for immunohistochemical analysis.

Furusawa Yoshikazu Y Takei Junko J Sayama Yusuke Y Yamada Shinji S Kaneko Mika K MK Kato Yukinari Y

Biochemistry and biophysics reports 20190428

Sensitive and specific monoclonal antibodies (mAbs) targeting podoplanin (PDPN) are needed for immunohistochemical analyses using formalin-fixed paraffin-embedded tissues because PDPN is known as a lymphatic endothelial cell maker in pathology. Recently, we established <i>anti</i>-PDPN mAbs against many species, such as human, mouse, rat, rabbit, dog, cat, bovine, pig, horse, goat, tiger, alpaca, and Tasmanian devil. However, anti-bear PDPN (bPDPN) has not been established yet. In this study, we ...[more]

PMID: 31061899

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