X-Ray Crystallographic Studies for Revealing Binding Sites of General Anesthetics in Pentameric Ligand-Gated Ion Channels.
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ABSTRACT:
SUBMITTER: Chen Q
PROVIDER: S-EPMC6490682 | biostudies-literature | 2018
REPOSITORIES: biostudies-literature
Publications
X-Ray Crystallographic Studies for Revealing Binding Sites of General Anesthetics in Pentameric Ligand-Gated Ion Channels.
Chen Qiang Q Xu Yan Y Tang Pei P
Methods in enzymology 20180324
X-ray crystallography is a powerful tool in structural biology and can offer insight into structured-based understanding of general anesthetic action on various relevant molecular targets, including pentameric ligand-gated ion channels (pLGICs). In this chapter, we outline the procedures for expression and purification of pLGICs. Optimization of crystallization conditions, especially to achieve high-resolution structures of pLGICs bound with general anesthetics, is also presented. Case studies o ...[more]
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