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First Report of Integrative Conjugative Elements in Riemerella anatipestifer Isolates From Ducks in China.


ABSTRACT: We report for the first time the occurrence of integrative conjugative elements (ICEs) in Riemerella anatipestifer (R.anatipestifer) isolated from diseased ducks in China. For this purpose, a total of 48 genome sequences were investigated, which comprised 30 publicly available R. anatipestifer genome sequences, and 18 clinical isolates genomes sequences. Two ICEs, named ICERanRCAD0133-1 and ICERanRCAD0179-1 following the classic nomenclature system, were identified in R. anatipestifer through the use of bioinformatics tools. Comparative analysis revealed that three ICEs in Ornithobacterium rhinotracheale showed a high degree of conservation with the core genes of ICERanRCAD0133-1, while 13 ICEs with high similarity to ICERanRCAD0179-1 were found in Bacteroidetes. Based on the definition of ICE family, ICERanRCAD0179-1 was grouped in CTnDOT/ERL family; however, ICERanRCAD0133-1, which had no significant similarity with known ICEs, might be classified into a novel ICE family. The sequences of ICERanRCAD0133-1 and ICERanRCAD0179-1 were 70890 bp and 49166 bp in length, had 33.14 and 50.34% GC content, and contained 77 CDSs and 51 CDSs, respectively. Cargo genes carried by these two ICEs were predicted to encode: R-M systems, IS elements, a putative TonB-dependent receptor, a bacteriocin/lantibiotic efflux ABC transporter, a tetracycline resistance gene and more. In addition, phylogenetic analyses revealed that ICERanRCAD0179-1 and related ICEs were derived from a common ancestor, which may have undergone divergence prior to integartation into the host bacterial chromosome, and that the core genes co-evolved via a related evolutionary process or experienced only a low degree of recombination events during spread from a common CTnDOT/ERL family ancestor. Collectively, this study is the first identification and characterization of ICEs in R. anatipestifer; and provides new insights into the genetic diversity, evolution, adaptation, antimicrobial resistance, and virulence of R. anatipestifer.

SUBMITTER: Zhu D 

PROVIDER: S-EPMC6491836 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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First Report of Integrative Conjugative Elements in <i>Riemerella anatipestifer</i> Isolates From Ducks in China.

Zhu Dekang D   Wan Jianbang J   Yang Zhishuang Z   Xu Jinge J   Wang Mingshu M   Jia Renyong R   Chen Shun S   Liu Mafeng M   Zhao Xinxin X   Yang Qiao Q   Wu Ying Y   Zhang Shaqiu S   Liu Yunya Y   Zhang Ling L   Yu Yanling Y   Chen Xiaoyue X   Cheng Anchun A  

Frontiers in veterinary science 20190424


We report for the first time the occurrence of integrative conjugative elements (ICEs) in <i>Riemerella anatipestifer</i> (<i>R.anatipestifer</i>) isolated from diseased ducks in China. For this purpose, a total of 48 genome sequences were investigated, which comprised 30 publicly available <i>R. anatipestifer</i> genome sequences, and 18 clinical isolates genomes sequences. Two ICEs, named ICE<i>Ran</i>RCAD0133-1 and ICE<i>Ran</i>RCAD0179-1 following the classic nomenclature system, were identi  ...[more]

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