Targeting the ICOS/ICOS-L pathway in a mouse model of established allergic asthma disrupts T follicular helper cell responses and ameliorates disease.

ABSTRACT:

Background

Allergic asthma is characterized by chronic inflammation and remodelling of the airways, associated with dysregulated type 2 immune responses and allergen-specific IgE. T follicular helper cells (T_FH ) are crucial in T-dependent B-cell responses and have been implicated in allergic airway disease (AAD). T_FH , unlike other CD4⁺ T cells, are uniquely reliant on continuous ICOS signalling to maintain their phenotype after T-cell priming; therefore, disrupting this signal can impair T_FH responses. However, the contribution of T_FH to disease during chronic aero-allergen exposure and the therapeutic potential of targeting these cells have not been evaluated.

Methods

To establish AAD, female BALB/c mice were repeatedly exposed to house dust mite or Alternaria alternata three times a week for up to 5 weeks. To examine the impact of T_FH on AAD, mice were allergen exposed for 5 weeks and co-administered anti-ICOS Ligand-targeted antibodies, three times a week for the last 2 weeks.

Results

T_FH were first observed in the lung-draining lymph nodes and with further exposure were also found locally within the lungs. T_FH accumulated with sustained allergen exposure, alongside germinal centre (GC) B cells. Blockade of ICOS signalling after AAD establishment successfully depleted T_FH but did not affect the differentiation of other CD4⁺ T-cell subsets. This reduced GC responses, allergen-specific IgE, inflammation, pulmonary IL-13 and airway hyper-responsiveness.

Conclusions

T_FH are crucial in the regulation of AAD and the ICOS/ICOS-L pathway could represent a novel therapeutic target in allergic asthma.

SUBMITTER: Uwadiae FI 

PROVIDER: S-EPMC6492018 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature