Ontology highlight
ABSTRACT:
SUBMITTER: Chassin H
PROVIDER: S-EPMC6494899 | biostudies-literature | 2019 May
REPOSITORIES: biostudies-literature
Chassin Hélène H Müller Marius M Tigges Marcel M Scheller Leo L Lang Moritz M Fussenegger Martin M
Nature communications 20190501 1
Tight control over protein degradation is a fundamental requirement for cells to respond rapidly to various stimuli and adapt to a fluctuating environment. Here we develop a versatile, easy-to-handle library of destabilizing tags (degrons) for the precise regulation of protein expression profiles in mammalian cells by modulating target protein half-lives in a predictable manner. Using the well-established tetracycline gene-regulation system as a model, we show that the dynamics of protein expres ...[more]