Expression phenotype changes of EBV-transformed lymphoblastoid cell lines during long-term subculture and its clinical significance.

ABSTRACT:

Objectives

The EBV-transformed lymphoblastoid cell line (LCL) is a useful resource for population-based human genetic and pharmacogenetic studies. The principal objective here was to assess expression phenotype changes during long-term subculture of LCLs, and its clinical significance.

Materials and methods

We searched for genes that were differentially expressed in 17 LCLs at late (p161) passage compared to early passage (p4) using microarray assay, then validated them by real-time RT-PCR analysis. In addition, we estimated correlations between expression phenotypes of 20 LCL strains at early passage and 23 quantitative clinical traits from blood donors of particular LCL strains.

Results

Transcript sequences of 16 genes including nuclear factor-kappaB (NF-kappaB) pat

SUBMITTER: Lee JE 

PROVIDER: S-EPMC6496229 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature